National League North and South clubs enter the fray and the dreams of Wembley can really begin.
It really is anyone's competition after Step 3 AFC Hornchurch's incredible achievement last May.
They left Hereford heartbroken under the Arch - and Josh Gowling's team will look to go again and begin with a very tough match at home to Kidderminster Harriers.
Two more big National League North promotion-hunters collide - Boston United will travel to Brackley Town.
It’s certainly not the only same-division tie - Chester head to Curzon Ashton, York City play Blyth Spartans and AFC Fylde face Gateshead.
Spennymoor and Chorley - both having a great season - will also cross swords.
It’s a similar story in the National League South.
Promotion rivals Oxford City and St Albans City are to play each other in the next round later this month.
Maidstone United face Billericay Town, Hungerford play Welling United and Bath City host Dartford.
Dorking Wanderers go to Step 3 Worthing while Ebbsfleet United face a tough task against Cray Wanderers.
The Second Round will take place on Saturday 27 November.
Click here for the full draw.