Just 32 clubs remain in the competition – 25 of whom ply their trade in the Vanarama National League.
Here are four fixtures to keep an eye on this weekend.
Spennymoor Town vs Plymouth Parkway
Plymouth Parkway face a gruelling excursion from the West Country as they hit the road for a 780-mile round trip to Spennymoor Town’s Brewery Field.
Parkway booked their unfamiliar visit to County Durham with a penalty shootout win over Dulwich Hamlet in the Third Round.
Spennymoor have enjoyed a more comfortable route to this point, beating Northern Premier League side Radcliffe 1-0 back in December.
Plymouth have won each of their last five in all competitions, but a string of postponements means they are yet to take to the field in 2022.
Yeovil Town vs Needham Market
Yeovil Town welcome Southern League Central opponents Needham Market to Huish Park.
The Marketmen have not lost on their travels in more than two months and got the better of National League side Wealdstone in their Third Round outing.
Having previously gone 11 matches without loss, Yeovil have hit a rough patch of late with four defeats from their last four.
After being a goal down to Woking in the Third Round, the Glovers turned the game around with an explosive final 10 minutes which saw them race to a 3-1 victory.
Dagenham & Redbridge vs Southend United
Dagenham & Redbridge clash with nearby neighbours Southend United at Victoria Road for the second time this season on Saturday.
The Daggers put their guests to the sword in league action with a 3-0 win back in October.
Dagenham reached the Fourth Round with a victory over Truro City on penalties, having conceded a late equaliser which left the well-travelled Londoners sat nervously for the finale.
It has been six years since Daryl McMahon’s side progressed beyond this stage.
Southend earned Saturday’s meeting with a comeback win over Dorking Wanderers. United are on their best form of the campaign, with three wins and a draw from their last four in all competitions.
Boreham Wood vs Maidstone United
National League South outfit Maidstone United must take to the M25 on Saturday for a bout with high-flying Boreham Wood.
The two sides met in the FA Trophy back in 2016, when Boreham Wood came from behind to win 3-2 in a replay of the Third Round.
The Wood have not been beaten at home this season and are on a fabulous run in all competitions, with a Trophy win over Barnet and an FA Cup victory over AFC Wimbledon each coming within the last five matches.
Maidstone beat Billericay Town in the Second Round before a walkover against Maidenhead put them into the current round.
Hakan Heyrettin's side have been in red-hot form of late and come into this tie unbeaten in 10 matches.
All of this weekend's FA Trophy Fourth Round ties:
Alfreton Town v FC Halifax Town
Dagenham & Redbridge v Southend United
Wrexham v Folkestone Invicta
St Albans City v Cheshunt
York City v Slough Town
Stourbridge v Guiseley
Tonbridge Angels v King's Lynn Town
Southport v Solihull Moors
Notts County v Eastleigh
Dartford v Weymouth
Aldershot Town v Bromley
Boreham Wood v Maidstone United
Morpeth Town v Boston United
Yeovil Town v Needham Market
Stockport County v Larkhall Athletic
Spennymoor Town v Plymouth Parkway