Winners of the Saturday February 2 matches will earn a cheque for £8,000.
Holders Brackley Town face a trip to Chesterfield as they look to make a Wembley return.
Heroes of the last round AFC Telford go back to the north east as they get on the road again and head to Spennymoor Town.
Leyton Orient, the National League leaders who beat Wrexham on Saturday, welcome in-form North side Blyth Spartans.
Salford City host Maidstone United, while the winners of the Solihull Moors and FC Halifax replay on Tuesday go to Hemel Hempstead.
Barnet and AFC Fylde face games against non-National League sides.
Bostik Premier Carshalton away is the Bees' task, while the Coasters must go to either Ramsbottom or Weymouth.
Stockport County's double dream lives on - and Manager of the Month Jim Gannon takes his team to Harrogate Town.
FA Trophy third round ties:
Leyton Orient v Blyth Spartans
Chesterfield v Brackley Town
Salford City v Maidstone United
Hemel Hempstead Town v Solihull Moors/FC Halifax Town
Harrogate Town v Stockport County
Spennymoor Town v AFC Telford United
Carshalton Athletic v Barnet
Ramsbottom United/Weymouth v AFC Fylde