Marc Richards, a former Tigers favourite as a player, has been charged with keeping the club from a return to the Southern League.
Gloucester have been without a manager since long-serving Tim Harris resigned his position at the end of November.
Richards, assistant manager under Harris and assistant director of Elite Sport at Hartpury College, says he is up for the challenging of steadying the ship.
“It’s obviously been an extremely difficult few weeks for everyone involved with the club with the recent departures and I can’t thank Tim enough for what he has done for me since my involvement with the club back in 2007," he said.
"When Tim decided to leave I needed time to reflect on what was best moving forward. I’m really excited to have the opportunity to manage Gloucester City AFC.
"The current squad of players have not shown their full potential yet this season so I’m really looking forward to seeing what they are capable of achieving not only this season but in the future. “
City have been hit by further issues after chairman Mike Dunstan confirmed he is to step away from the club before the New Year.
Gloucester have struggled for form since switching from the National League North to the South in the close season.
They sit 18th in the table, just two points outside the relegation zone and the new man’s first game in charge will get a trip to Truro City next Saturday.