It all starts for the National League North and South clubs who become involved for the first time.
Click here for the full draw.
There are some fantastic ties in prospect for Saturday 22 September, with prize money of £9,000 on offer.
Chester will have a derby game to enjoy with City of Liverpool visiting.
In possibly the tie of the round, Stockport County will travel to ambition Evo-Stik NPL club South Shields - and a big crowd will be expected.
Step 5 side AFC Uckfield Town will play National League South Dartford at The Oaks, while Weymouth or Banbury will host Bath City.
North plays South as Hereford welcome Truro City while Torquay United will go to either Lymington Town or Lydney Town, who will both be desperate for a huge scalp.
There's an all-North encounter as Darlington face Bradford (Park Avenue).
North leaders Chorley will be happy enough with a home game with either Parkgate or Leek Town.