The 2019/20 campaign will be formed of two regional divisions, twelve Clubs in the South and ten in the North, with sides playing each other twice, both home and away.
Clubs will also participate in an additional cup competition later in the season.
Licences were granted to those who had met The National League’s criteria in mid-August.
Speaking at the time of Club License confirmation, Head of Player Development, Gary Owers, said: “The National League and National League Youth Alliance is committed to Youth Development.
"As part of our ongoing strategic approach we have implemented a scheme for Clubs to demonstrate their own commitment to raising and maintaining standards in this area.
“The aim of a National League Football Academy is to provide an environment and player pathway which allows Clubs to develop and produce more and better home grown players”.
Matchday One Fixtures
Boston United vs AFC Fylde
Guiseley vs FC Halifax Town
Hartlepool United vs Alfreton Town
Solihull Moors vs Darlington
York City vs Chester
Boreham Wood vs Woking
Bromley vs Aldershot Town
Dartford vs Chippenham Town
Dover Athletic vs Weston Super Mare
Hampton & Richmond Borough vs Oxford City
Sutton United vs Maidenhead United